Terminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity

To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer,…

WeiterlesenTerminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity

Uncovering a Novel Molecular Mechanism for Scavenging Sialic Acids in Bacteria

Neu5Ac (blue), 2,7-anhydro-Neu5Ac (orange), or XY intermediate (gray). B and C, superposition of the 1H,13C HSQC reference spectra of Neu5Ac (blue) and 2,7-anhydro-Neu5Ac (orange) and 1H,13C HSQC reaction mixture at…

WeiterlesenUncovering a Novel Molecular Mechanism for Scavenging Sialic Acids in Bacteria